Sesefa se thibelang likokoana-hloko haholo-holo se nka ntho e nyane e nang le sebaka se seholo se ikhethileng le mokhoa o nepahetseng oa ho sefa oa 0.22 μm kapa ka holimo.E sebelisoa haholo-holo ho thibela litšila tse moeeng le libaktheria tse kotsi le likokoana-hloko ho kena ka tanka, mohala oa tlhahiso, Aseptic Chamber, joalo-joalo, liphetoho tsa boleng ba metsi, sehlahisoa le tikoloho e sa hloekang ea kamore, lisebelisoa tsa filthara li entsoe ka thepa ea hydrophobic polytetrafluoroethylene. molomo, ka mor'a hore coagulation karohano ho hlahisa sterile Compressed air.Moetso oa ts'ebeliso ke sesefa se loketseng sa khase ea organic solvent se sebelisetsoang ho tlosa libaktheria.Haholo-holo e sebelisoa ka meriana, ho belisoa, lijo le seno, ho riteloa biri, lihlahisoa tsa likokoana-hloko le liindasteri tse ling ho kopana le lijo, lik'hemik'hale, lino, biri, meriana, lisebelisoa tsa elektronike le liindasteri tse ling.